Mohd Farid Laporan Latihan Industri Sarjana Muda Sains Bioperubatan UKM Tahun 3 Sesi 2007/2008-Hospital Selayang Part V-Blood And CSF Bench

BLOOD AND CSF BENCH

BLOOD SPECIMEN

Purpose: To identify a bacteria and fungi which cause septicemia as a result of intravascular or extravascular infections

Screening Method

Using an automatic instrument, the BACTEC 9240. Blood is inoculated into the vial, which is then inserted into the instrument for incubation and periodic reading. Growth is detected in the broth by CO₂ production as each vial contains a sensor which can detect increases in CO₂ produced by the growth of microorganisms. The sensor is monitored by the instrument system minutes for an increase in its fluorescence that is proportional to the amount of CO₂ present. A positive reading indicates the presumptive presence of viable microorganisms in the bottle.

• Identification of microorganism
• Gram staining
• Blood agar
• MacConkey Agar
• Chocolate Agar
• Sabouraud Dextrose Agar
• Anaerobic Blood Agar

CEREBROSPINAL FLUID

Purpose: For detection of microorganisms which cause bacterial and fungal meningitis

Method

Describe the appearance of the sample

Eg: clear, turbid, xantochromic or bloodstain

Perform cell count

5 drop of CSF and add 5 drop of toluidine blue in a test tube.

Fill Nuebauer chamber with diluted CSF and wait for 3 minutes.

Count the cells in 4 of the large square in Nuebauer Chamber

If blood stained sample, we unable to perform cell count.

Culture

Blood Agar

MacConkey Agar

Chocolate agar- incubate 48hour in carbon dioxide incubator at 35 to 37^oC

Sabouraud Dextrose Agar – incubate 24 hours at 30 ^oC

Examine microscopically

Gram staining-bacterial morphology

India ink- Cryptococcus sp.

Biochemical Testing

According to bacteria that grow on the media agar

Antibacterial Susceptibility testing

Bacterial Antigen Test